technique for particle analysis

[Lee S]

Dear,

I am a beginer in particle analysis to measure the pixel area and the number
of particles.
Enclosed is an example.
The island parts in white color should be treated as an indepent particle.
But my ImageJ figured out only two small dots as particles.

Would you please give a comment how to manipulate it?

Best,

Lee

[Christine Labno]

Dear Lee,

I could get ImageJ to see only the two "islands" if I first
used Image --> Lookup Tables --> Invert LUT to make the
islands black = 255 instead of white = 255.  I also checked
"exclude on edges" during the Analyze particles step.

Best,
Christine


---- Original message ----
>Date: Fri, 6 Nov 2009 13:33:05 -0500
>From: Lee S <[hidden email]>  
>Subject: technique for particle analysis  
>To: [hidden email]
>
>Dear,
>
>I am a beginer in particle analysis to measure the pixel area
and the number
>of particles.
>Enclosed is an example.
>The island parts in white color should be treated as an
indepent particle.
>But my ImageJ figured out only two small dots as particles.
>
>Would you please give a comment how to manipulate it?
>
>Best,
>
>Lee
>________________
>test.tif (67k bytes)
Christine Labno, Ph.D.
Asst. Technical Director
Light Microscopy Core
University of Chicago
Office of Shared Research Facilities
Abbott 129
[phone] (phone)

Please consider the environment before printing this email.

[Gabriel Lapointe-2]

Hi Lee,
To count white particles you first have to apply some form of
thresholding with the "dark background" option check. Also, if you want
to include the white "blob" at the edge of the image, then make sure
that the "Exclude on Edges" option is unchecked.

Regards,
Gabriel

Gabriel Lapointe, MSc.
Laboratoire de Luc DesGroseillers, PhD.
Pavillon Roger-Gaudry Local A-538
Département de biochimie
Faculté de Médecine de l'Université de Montréal
2900 boul. Édouard-Montpetit,
Montréal, Qc, H3T 1J4
Tel : [phone] postes 5187, 5152, 5162 ou 1048
Fax : [phone]
[hidden email]


On Fri, 2009-11-06 at 13:33 -0500, Lee S wrote:

> Dear,
>
> I am a beginer in particle analysis to measure the pixel area and the number
> of particles.
> Enclosed is an example.
> The island parts in white color should be treated as an indepent particle.
> But my ImageJ figured out only two small dots as particles.
>
> Would you please give a comment how to manipulate it?
>
> Best,
>
> Lee